Table. 4c. (c). Robustness – change in flow rate.
| System suitability | Compound | Change in flow rate | |||||
|---|---|---|---|---|---|---|---|
| (A) Normal condition | (B) 1.2 mL/minutes | (C) 1.4 mL/minutes | |||||
| Mean (n = 6) | RSD (%) | Mean (n = 6) | RSD (%) | Mean (n = 6) | RSD (%) | ||
| Retention time, tR (minutes) |
QUE | 3.972 | 0.175 | 4.291 | 0.105 | 3.696 | 0.130 |
| BDMC | 13.868 | 0.310 | 14.953 | 0.321 | 12.909 | 0.333 | |
| DMC | 15.255 | 0.265 | 16.442 | 0.284 | 14.235 | 0.279 | |
| CUR | 16.743 | 0.213 | 18.038 | 0.262 | 15.668 | 0.298 | |
| Peak area | QUE | 7039483 | 0.562 | 7606272 | 0.662 | 6530571 | 0.497 |
| BDMC | 180475 | 0.541 | 194216 | 0.753 | 167111 | 1.593 | |
| DMC | 1000716 | 0.736 | 1078076 | 0.714 | 928707 | 1.345 | |
| CUR | 3433379 | 0.754 | 3700134 | 0.690 | 3185325 | 1.198 | |
| Resolution, R | QUE | - | - | - | - | - | - |
| BDMC | 32.327 | 0.172 | 32.779 | 0.199 | 32.047 | 0.928 | |
| DMC | 2.900 | 0.370 | 2.921 | 0.608 | 2.936 | 2.014 | |
| CUR | 2.840 | 0.429 | 2.864 | 0.723 | 2.868 | 1.647 | |
| Tailing factor, Tf | QUE | 1.366 | 0.077 | 1.360 | 0.183 | 1.371 | 0.287 |
| BDMC | 1.493 | 1.377 | 1.490 | 1.891 | 1.539 | 1.614 | |
| DMC | 1.160 | 1.075 | 1.157 | 1.447 | 1.181 | 2.364 | |
| CUR | 1.085 | 0.148 | 1.081 | 0.101 | 1.087 | 0.207 | |
| Theoretical plate, N | QUE | 8711.993 | 0.267 | 9148.347 | 0.429 | 8249.430 | 0.420 |
| BDMC | 15740.557 | 0.397 | 16035.103 | 1.342 | 15696.046 | 2.851 | |
| DMC | 14041.181 | 0.701 | 14374.944 | 1.036 | 13420.220 | 0.844 | |
| CUR | 15793.019 | 0.472 | 16216.013 | 1.854 | 15379.165 | 2.364 | |
| Capacity factor, k’ | QUE | 0.656 | 1.783 | 0.661 | 0.832 | 0.627 | 0.762 |
| BDMC | 4.798 | 1.202 | 4.780 | 0.942 | 4.750 | 3.067 | |
| DMC | 5.351 | 0.661 | 5.355 | 0.497 | 5.350 | 2.846 | |
| CUR | 5.966 | 0.632 | 5.985 | 0.500 | 5.862 | 0.427 | |