Table. 4. Serum biochemical values for rats in the sub-acute toxicity study for the control group and the groups treated with different doses of the test extract of W. volubilis.

Parameters	Vehicle	125 mg/kg	250 mg/kg	500 mg/kg
ALT	26.32 ± 0.83	27.62 ± 0.13	28.12 ± 0.72	28.89 ± 0.43
ALP	26.11 ± 0.17	26.87 ± 0.03	28.12 ± 0.84	29.46 ± 0.83
TP	6.84 ± 0.14	8.14 ± 0.08	8.39 ± 0.15	8.41 ± 0.18
Bilirubin	0.33 ± 0.02	0.38 ± 0.04	0.40 ± 0.01	0.58 ± 0.03
Total cholesterol	49.55 ± 0.28	53.85 ± 0.64	55.35 ± 1.14	59.12 ± 1.52
Triglycerides	52.16 ± 1.35	55.46 ± 1.54	57.96 ± 1.26	64.16 ± 1.04
Urea	16.33 ± 0.68	18.63 ± 0.97	18.83 ± 0.37	20.33 ± 0.53
Creatinine	1.58 ± 0.04	1.71 ± 0.05	1.42 ± 0.03	1.73 ± 0.04
Sodium	108.42 ± 1.72	109.72 ± 1.40	112.22 ± 1.13	119.42 ± 1.26
Potassium	1.69 ± 0.08	2.99 ± 0.04	3.24 ± 0.04	3.26 ± 0.06
α-amylase	113.64 ± 1.87	114.94 ± 0.14	117.44 ± 2.73	125.64 ± 1.23
Glucose	70.53 ± 0.87	71.83 ± 1.13	74.33 ± 2.09	74.53 ± 0.83

Data are expressed as means ± SEMs, n = 6, for each group. No statistical difference was found between the control group and the test groups treated with extract of W. volubilis (P < 0.05). ALT, alanine aminotransferase; ALP, alkaline phosphatase; TP, total serum protein.