Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Aug 20;5:44. doi: 10.1186/2040-2392-5-44

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Germain et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Quantitative reverse transcription PCR (qRT-PCR) validation of mRNA-Seq data. (a) Differential gene expression of select autism candidate genes and genes implicated in seizure disorders were verified by qRT-PCR in 10-week-old AS (AS del 1-0), normal (Nml 1-0), and idic(15) (Idic1-8) iPSC-derived neurons. P values: *P ≤0.1, **P ≤0.05, ***P ≤0.01. (b) Correlation of relative expression levels for 19 genes in iPSC-derived neurons as determined by mRNA-Seq and qRT-PCR. The log2-transformed RQ values for mRNA expression relative to normal neurons are plotted on the x-axis against the log2-transformed FPKM values obtained by mRNA-Seq analysis (y-axis).