Fig. 1.
Raloxifene induces cell death and decreases cell viability in MCF-7 cells. (A) MCF-7 cells were treated with 10 μM or 20 μM raloxifene (RAL) for 48 h. Cell viability was assessed using the MTS assay (mean ± SD; n = 3). *P < 0.05 compared to control. (B) Cell death was evaluated using the trypan blue exclusion assay after treatment with raloxifene for 48 h (mean ± SD; n = 3). *P < 0.05 compared to control. (C) MCF-7 cells were treated with 10 μM raloxifene for 24 or 48 h. Cell morphology was examined using a light microscope (Magnification, 20×; Scale bar, 50 μm).