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Forced expression of CCND1 rescued miR-576-3p induced G1 phase arrest and inhibition of colony formation. (A) T24 cells were co-transfected with both miR-576-3P mimic or NC and pReceiver-CCND1 or empty pReceiver vector. The expression of CCND1 or GAPDH was detected by Western blotting analysis. (B, C, and D) Forced expression of CCND1 partly restored cell cycle arrest effect and inhibition of colony formation rate of miR-576-3p in T24 cells (*P < 0.05).
