Fig. 4.
Protective effects of GmTRX under oxidative stress. (A) Effects of GmTRX on Arabidopsis growth under heat shock (HS) conditions. Seven-day-old wild-type (Col-0), GmTRX-expressing, and GmTRXdm-expressing Arabidopsis seedlings were subjected to heat treatment at 45°C for 2 h and allowed to recover at 22°C for 5 days. Survival rates of wild-type (Col-0), GmTRX-expressing, and GmTRXdm-expressing Arabidopsis plants after heat shock (HS) treatment are shown. (B) The 3,3′-diaminobenzidine (DAB) staining for H2O2 accumulation in 2-week-old seedlings of wild-type (Col-0), GmTRX-expressing, and GmTRXdm-expressing Arabidopsis under heat shock, H2O2 and menadione (Men) treatment conditions. (C) ROS accumulation in Arabidopsis protoplasts. After treatment with heat shock, H2O2, and menadione (Men), protoplasts were kept at 22°C for about 1 h and then subjected to 5 μM H2DCFDA for 10 min. DCF fluorescence images were visualized by LCSM and the fluorescence intensities were measured with a fluorescence spectrometer (excitation at 488 nm, emission at 500–600 nm). (D) Effects of GmTRX on uricase activity under oxidative stress and heat stress. Uricase activity was measured using total proteins from wild-type (Col-0), GmTRX-expressing, and GmTRXdm-expressing Arabidopsis treated with HS, H2O2, or menadione.