Fig. 1.

The sixth RNA pool that binds prostatic acid phosphatase (PAP) was enriched. (A) After six rounds of SELEX, the RNA ligands that bound PAP were enriched 3.52-fold using real-time RT-PCR. (B) The electrophoretic mobility shift assay (EMSA) results showed that the sixth RNA pool bound to the target proteins in a concentration-dependent manner compared to that of library. (C) Seventeen RNA aptamer candidates were obtained after a cloning and sequence analysis of the sixth RNA pool. These RNA aptamer candidates were classified into three groups according to their sequence similarities (Group I, Group II, and Non-Group). Identical nucleotides are represented as a line.