Table 1.
Characteristics of 22 EST-SSR loci for use in the genus Thujopsis.
| DDBJ accession no. | ||||||
| Locus | Primer sequences (5′–3′) | Repeat motif | Ta (°C) | Allele size range (bp) | 1a | 2b |
| Tdest1 | F: GCCTCCCTCGCGCCATCAGGATTTTCTGACAGGCTTTGTTCTC | (CT)11 | 60 | 137–173 | LC010288 | LC010310 |
| R: GTTTCTTAATTCCCAAGAGTGCTTATGAGTTC | ||||||
| Tdest3 | F: GCCTCCCTCGCGCCATCAGCGGCCCAGGTTTCTGTACTC | (AT)11 | 60 | 169–186 | LC010289 | LC010311 |
| R: GTTTCTTGCCCATTAAAGTCGGGTATTG | ||||||
| Tdest11 | F: GCCTCCCTCGCGCCATCAGTGGGATACATACTGCATTTGTTAGG | (AT)12 | 60 | 138–161 | LC010290 | LC010312 |
| R: GTTTCTTCTCCCCAAGCAAGTCACCAC | ||||||
| Tdest14 | F: GCCTCCCTCGCGCCATCAGCAGTAGACAATTTCTGCAAATCACC | (AG)12 | 60 | 157–185 | LC010291 | LC010313 |
| R: GTTTCTTTCCCTTTTGTTGGCATTATAGG | ||||||
| Tdest17 | F: GCCTCCCTCGCGCCATCAGGCTTTTGATGTCCGCTATATCCTC | (AG)12 | 60 | 159–168 | LC010292 | LC010314 |
| R: GTTTCTTGGAGATTCCAATGTTTGTCATGC | ||||||
| Tdest21 | F: GCCTCCCTCGCGCCATCAGGTCCATCCATTCTCACTCCAAAG | (AG)13 | 60 | 231–274 | LC010293 | LC010315 |
| R: GTTTCTTAGCAGACCCTATTTCACAGCATC | ||||||
| Tdest24 | F: GCCTCCCTCGCGCCATCAGATACCATACAGCTTTCAGCCAG | (AT)15 | 60 | 243–267 | LC010294 | LC010316 |
| R: GTTTCTTGCAGAACAAACGAATCAATGAGAG | ||||||
| Tdest29 | F: GCCTCCCTCGCGCCATCAGAAACGACTCTGCTGGATTTCAC | (AC)16 | 60 | 219–246 | LC010295 | LC010317 |
| R: GTTTCTTTTCCGCTCTTGATTTTCTCTCC | ||||||
| Tdest35 | F: GCCTCCCTCGCGCCATCAGAAGCTATTGACCCTTCTCAGGATAC | (CT)15 | 60 | 194–230 | LC010296 | LC010318 |
| R: GTTTCTTCCATGTTGAATTGTTCCCTTTC | ||||||
| Tdest37 | F: GCCTCCCTCGCGCCATCAGCCAAGCGACAGAAAACCATTC | (ATC)9 | 60 | 164–176 | LC010297 | LC010319 |
| R: GTTTCTTTCAGTCTCTTCCTCCTCCTCCTC | ||||||
| Tdest38 | F: GCCTCCCTCGCGCCATCAGTGACCATTCCTCCTCCTCCTC | (ACC)9 | 60 | 117–134 | LC010298 | LC010320 |
| R: GTTTCTTCATGTTTGCAGTTGAGAGAAGACC | ||||||
| Tdest39 | F: GCCTCCCTCGCGCCATCAGGCAGCACAGGAGAAGAAAGATG | (GCT)9 | 60 | 153–165 | LC010299 | LC010321 |
| R: GTTTCTTACAACAGCCACAACGTGTCC | ||||||
| Tdest42 | F: GCCTCCCTCGCGCCATCAGCTCCCTATCCCAACACCAACAC | (ACC)9 | 60 | 226–255 | LC010300 | LC010322 |
| R: GTTTCTTTGCCTACCTATCCTTCTTCTTCTCC | ||||||
| Tdest43 | F: GCCTCCCTCGCGCCATCAGGGTCCAATGCAGGTAATACAAGAAG | (CGG)9 | 60 | 137–153 | LC010301 | LC010323 |
| R: GTTTCTTTCCCCGCCAAGATACTCAAC | ||||||
| Tdest44 | F: GCCTCCCTCGCGCCATCAGTTTGGTGGTGGAGGTGGTG | (GAT)9 | 60 | 134–137 | LC010302 | LC010324 |
| R: GTTTCTTCGCTTATGCCAAGCAGTCATC | ||||||
| Tdest45 | F: GCCTCCCTCGCGCCATCAGTGAGGGTGGTGAGACAATTC | (GGT)12 | 60 | 211–236 | LC010303 | LC010325 |
| R: GTTTCTTCAAGATTTGGAACTCCTGCAAC | ||||||
| Tdest49 | F: GCCTCCCTCGCGCCATCAGGTGCCCTCAAAGTTACAGCAGTC | (GAT)10 | 60 | 233–248 | LC010304 | LC010326 |
| R: GTTTCTTGCAATCACCTCATCCTCACTTC | ||||||
| Tdest52 | F: GCCTCCCTCGCGCCATCAGTTCAGGAAGGCCAAGGAGAG | (GGT)11 | 60 | 239–251 | LC010305 | LC010327 |
| R: GTTTCTTGATCCTCCTGCATCATTTTGTTC | ||||||
| Tdest53 | F: GCCTCCCTCGCGCCATCAGCCAAAGCCCTTCCAGTAACATC | (CTT)13 | 60 | 244–284 | LC010306 | LC010328 |
| R: GTTTCTTGATGGAATGAGTGAATCTCAGGAAC | ||||||
| Tdest54 | F: GCCTCCCTCGCGCCATCAGCCCTGTATTATTCTCAACATCATCG | (CTT)11 | 60 | 182–203 | LC010307 | LC010329 |
| R: GTTTCTTGGGATTCAGACAAGGGCAAG | ||||||
| Tdest56 | F: GCCTCCCTCGCGCCATCAGCATTGCCCTTTGGAATATAGGATC | (AAG)9 | 60 | 153–165 | LC010308 | LC010330 |
| R: GTTTCTTGTTGCCCATCTGCTCTTCTTC | ||||||
| Tdest58 | F: GCCTCCCTCGCGCCATCAGCTGAACGGCGCCCTAATCTC | (AAG)13 | 60 | 151–180 | LC010309 | LC010331 |
| R: GTTTCTTGCCCACTCCTCAAATCCAAC | ||||||
Note: DDBJ = DNA Data Bank of Japan; Ta = annealing temperature.
a
1 = T. dolabrata var. hondae.
b
2 = T. dolabrata.