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. 2015 Jan 21;11(1):775. doi: 10.15252/msb.20145504

Figure 6.

Figure 6

Overview of JUN/CREB/ATF/NFATC1 subnetwork
  1. JUN/CREB/ATF/NFATC1 subnetwork map. Arrows indicate the identifications from TAP/MS. Bold arrows indicate the identifications from both TAP/MS and endogenous AP. Colours of arrows indicate the locations of interactions: red, in chromatin only; purple, in both fractions.
  2. NFATC1 binds to other factors mainly in chromatin fractions. 293T cells were transfected with constructs encoding MYC-tagged NFATC1 and SFB-tagged other TFs as indicated. Pull-down experiments were carried out with S-protein beads and immunoblotted with antibodies as indicated.
  3. ATF1 binds to ATF2, JUN and CREB1 in both fractions. 293T cells were transfected with constructs encoding MYC-tagged ATF1 and SFB-tagged other TFs as indicated. Pull-down experiments were carried out with S-protein beads and immunoblotted with antibodies as indicated.
  4. Overlap Venn diagram of CREB1, ATF2 and NFATC1 target genes using ChIP-seq data sets generated by the ENCODE consortium in GM12878 cells.

Source data are available online for this figure.