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Phosphorylation of Akt, AS160, and TBC1D1 in rat soleus muscles 2 h after treadmill exercise (180 min at 9 m/min). See the Materials and Methods section for details. Frozen muscles were used to measure the phosphorylation of Akt Ser473 (A), Akt Thr308 (B), AS160 Thr642 (C), AS160 Ser588 (D), TBC1D1 Thr590 (E), and TBC1D1 Ser237 (F). Values are mean ± SE, (A–D) n = 7–8; (E) n = 6–7; (F) n = 7–8. †P < 0.05 versus 0 μU/mL insulin. *P < 0.05 versus resting with same insulin concentration.
