Table 3.
Effects of PDTC on renal NF‐κB expression and activity in Lewis and LPK rats.
| Parameter | Lewis+V | Lewis + P(40 × 2) | LPK+V | LPK+P(40 × 2) |
|---|---|---|---|---|
| %p50 stain/tissue | 58.7 ± 12.3 | 59.6 ± 9.3 | 50.5 ± 9.6 | 55.1 ± 3.7 |
| %p50 stain/area | 52.6 ± 13.7 | 54.5 ± 9.5 | 27.0 ± 7.6 | 27.6 ± 3.5 |
| p50 protein (fold‐increase above Lewis+V) | 1.00 ± 0.23 | 1.34 ± 0.18 | 2.30 ± 0.77* | 2.23 ± 0.85 |
| p65 transcription binding (A450) | 1.00 ± 0.09 | 1.00 ± 0.10 | 0.56 ± 0.25* | 0.73 ± 0.22 |
A450; absorbance value at 450 nm; LPK, Lewis polycystic kidney; P(40 × 1), PDTC 40 mg/kg once daily; P(40 × 2), PDTC 40 mg/kg twice daily; V, vehicle.
Expression of p50 protein was assessed by immunohistostaining and by Western blot. Protein:DNA binding activity was assessed by p65 transcription binding assay. Data expressed as mean ± SD.
*P < 0.05 versus Lewis + V. In all experiments: Lewis + V (n = 3) and Lewis + P (n = 4). For Western blotting and p65 binding experiments: LPK + V (n = 7–9), LPK + P(40 × 2) (n = 7–9). For p50 immunohistochemistry experiments: LPK + V (n = 4), LPK + P(40 × 2) (n = 4).