Na, K‐ATPase in renal cortex from WT and
Fxyd2−/− mice. (A). Representative Western blot
shows staining with K3 antibody to detect Na,K‐ATPase α1 and
β1 subunits in cortical membranes. Equal amounts of protein (5 μg)
were loaded per lane, n = 6 for each genotype. Staining with antibody
against FXYD2b (RNGB) was used to verify mouse genotype. (B) Immunofluorescence staining forβ
β1 subunit of Na,K‐ATPase. PT, proximal convoluted tubules, DCT,
distal convoluted tubules, G, glomerulus. Intensity of stain was higher in DCT than in PT, and
similar for both genotypes. (C) Na,K‐ATPase activity was assayed from membranes of WT (closed
circles) and Fxyd2−/− mice (closed squares)
(n = 6 for each genotype). Ouabain‐dependent ATP hydrolysis
(Vmax, μmol Pi/mg h) is expressed as means ± SEM.
The asterisk indicates statistical significance (P < 0.05).