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Basal expression and phosphorylation of NKCC2 transporter. Cortical membrane fractions from WT and Fxyd2−/− mice were analyzed by Western blots with the antibodies against total (A) and phosphorylated NKCC2 (C). Blots were scanned and the density values were normalized to mean density of the WT group for total (B) and pNKCC2 (D). The data are expressed as means ± SEM. Asterisk indicates statistical significance (P < 0.01).
