Figure 1.

Identification of h2‐calponin in human (A, B) and mouse platelets (C, D). (A, C): Platelets, leukocytes, and erythrocytes were prepared on slides to assess for contamination. Lysates from platelet preparations were resolved on SDS‐PAGE along with erythrocyte and leukocyte lysates (internal negative and positive controls, respectively). H2‐calponin was probed (RAH2 primary antibody and, alkaline phosphate‐labeled anti‐rabbit IgG second antibody (B, D): Platelets, leukocytes, and erythrocytes were examined by immunofluorescence. H2‐calponin was visualized using RAH2 antibody followed by FITC‐conjugated second antibody (central column). Normal rabbit serum (NS) was used as primary antibody control (right column). Bright‐field images (left column) were obtained using a differential interference contrast (DIC) filter. Size bars indicate 10 μm.