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. 2014 Dec 18;2(12):e12240. doi: 10.14814/phy2.12240

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© 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Two different gels; one for TRPC1 and TRPC4 (top) and another for TRPC5 (bottom) isoforms, run under the same conditions. RT‐PCR for TRPC 1, 4, and 5 identifying three bands at a 238, 168, and 247 bp. On the top columns 1 and 2 total mRNA obtained from isolated juxtaglomerular (JG) cells. Column 3 is the “no‐template” negative control. Column 4 is the calibration scale (100 bp ladder). On the bottom column 1 is total mRNA obtained from isolated JG cells. Column 2 is the “no‐template” negative control and column 3 is the calibration scale (100 bp ladder).