Figure 5.

Effect of hypoxia–reoxygenation on the expression of CBS, CSE and cytokines in tubular cells. Human proximal tubular cells were subjected to 2‐h hypoxia followed by 24‐h reoxygenation (HR). Cells cultured in the regular medium without being subjected to HR were used as a control. A real‐time PCR analysis was used to measure the mRNA of CBS and CSE (A) and cytokines (MCP‐1, IL‐6) (B). Results are expressed as mean ± SEM (n = 5 for each group). *P < 0.05 when compared with the value obtained from control cells.