Figure 6.

Effect of CBS and CSE inhibitors on proinflammatory cytokine expression and glutathione levels in tubular cells. Human proximal tubular cells were incubated in the absence (control) or presence of a CBS inhibitor aminooxyacetic acid (AOAA, 0.2 mmol/L) or a CSE inhibitor DL‐propargylglycine (PAG, 0.5 mmol/L). The mRNA of MCP‐1 and IL‐6 was measured by a real‐time PCR analysis (A). Total intracellular glutathione levels were measured (B). Results are expressed as mean ± SEM (n = 5 for each group). *P < 0.05 when compared with the value obtained from control cells.