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. Author manuscript; available in PMC: 2015 Dec 15.
Published in final edited form as: J Immunol. 2014 Nov 3;193(12):6005–6015. doi: 10.4049/jimmunol.1401871

FIGURE 8.

FIGURE 8

Ligands for Siglec-E are induced during T cell maturation. Thymocytes and splenocytes from Rag1-GFP WT (grey filled histogram) or Rag1-GFP CD4-cre NKAP cKO (black open histogram) mice were stained with Siglec-E Fc fusion protein. Mouse Siglec-E preferrentially binds to α2,8-linked sialic acids over α2,3- or α2,6-linkages. Cell populations were defined as in Fig. 2. Below the FACS plots, the average MFI with SEM for WT and CD4-cre NKAP cKO mice is shown for each population. For thymic analysis, 3 WT and 4 CD4-cre NKAP cKO mice were analyzed for each population. For the splenic analysis, 5 WT and 4 CD4-cre NKAP cKO mice were analyzed for each population. Statistical analysis of the differences in MFI was performed using student’s t test.