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. 2015 Feb 3;12:23. doi: 10.1186/s12974-015-0248-1

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© Di Liddo et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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ENSc and formation of neurospheres. (A) After 1 day of culture, standard medium promoted the formation of neurospheres as evidenced by optical microscopy analysis. In contrast, several fibroblastoid colonies were observed in ENS cultures maintained in BM ENS medium. Bar: 100 μm. (B) Quantitative analysis of number and diameter of neurospheres obtained by LPS (5 μg/mL) or Wnt3a (20 ng/mL) stimulation. In parallel, the analysis was performed on LPS- and Wnt3a-untreated samples that were used as control. Light microscopic pictures reflected the increased number of neurospheres treated with LPS and Wnt3a after 1 (T1), 7 (T7), and 14 (T14) days in comparison to untreated cultures (control). (C) Data were quantified and normalized to controls (100%) (n = 3). (D) Diameter of neurospheres at T1, T7, and T14 was measured (n = 150) using ImageJ software. Statistical significance compared to control; p value ≤0.05*. Bar: 100 μm.