Figure 4.

Elution profile of anion-exchange chromatography (QAE-Toyopearl) loaded on the cell extract of P. alcaliphila AL15-21^T grown at pH 10 under low aeration intensity. The cell extract was prepared by cell disruption using a French press. The first (peak 1) and second (peak 2) peaks indicate cytochrome c-552 and a mixture of cytochromes c other than cytochrome c-552 (cytochrome c-554 and c-551), respectively. The percentages relative to total cytochromes c of peaks 1 and 2 are 64% and 36%, respectively.