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. 2015 Feb 13;10(2):e0113130. doi: 10.1371/journal.pone.0113130

Fig 1. GPR40 expression in different cell lines.

Fig 1

(A) The protein expression of GPR40 was most abundant in the renal tubule epithelial cell line LLCPKcl4 but very low in HEK293 cells, as revealed by immunoblotting a rabbit anti-human GPR40 antibody (Epitomics, Burlingame, CA) that also cross-reacts with monkey, porcine, and mouse GPR40. Shown was a representative blot from at least three separate experiments with similar results. (B) RT-PCR demonstrated that GPR40 mRNA expression was higher in LLCPKcl4 cells, with minimal expression in HEK293 cells.