Fig 4. Effect of human GPR40 transfection on EET-induced phosphorylation of EGFR and ERK in HEK293 cells.

Compared to empty vector-transfected HEK293 cells, the clones stably overexpressing human GPR40 exhibited markedly augmented phosphorylation of both EGFR and ERK in response to 14,15-EET (5 μM for 15 min). Shown are representative blots of 3 separate experiments with similar results and the quantitative analysis by densitometry from 3 independent experiments.