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. 2015 Feb 13;10(2):e0113130. doi: 10.1371/journal.pone.0113130

Fig 5. Effect of EGFR or HB-EGF inhibition on EET-stimulated phosphorylation of EGFR and ERK in HEK293 cells.

Fig 5

Quiescent HEK293 cells were treated with 5 μM 14,15-EET for 15 min. Human GPR40 (hGPR40) overexpression markedly augmented EET-induced phosphorylation of EGFR and ERK, which were inhibited by pretreatment of the cells with the EGFR tyrosine kinase inhibitor, AG1478 (A) or the HB-EGF inhibitor, CRM197 (B). Shown are representative blots from 1 of 3 separate experiments with similar results. *p<0.05 compared with vehicle alone in hGPR40 transfected HEK293 cells. #p<0.05 compared with 14,15-EET treated cells in hGPR40 transfected HEK293 cells.