Skip to main content
. 2015 Feb 13;10(2):e0116497. doi: 10.1371/journal.pone.0116497

Fig 1. Oligomerisation of α-syn on treatment with DA.

Fig 1

A. Schematic of the different α-syn constructs used in this study. The black bars represent the imperfect repeats (residues 10–16, 21–27, 32–37, 43–49, 57–63, 80–86). NAC region (stippled bar, residues 60–95). B. Silver stain SDS-PAGE gel of truncated α-syn incubated in the presence or absence of DA. Lane 1: α-syn 1–140. Lane 2: α-syn 1–140 + DA. Lane 3: α-syn 1–95. Lane 4: α-syn 1–95 + DA. Lane 5: α-syn 43–140. Lane 6: α-syn 43–140 + DA. The α-syn to DA ratio was 1:10. C. Size exclusion chromatography of DA:α-syn 43–140 and DA:α-syn 1–95 oligomers. 200 μM α-syn was incubated with 2 mM DA for 7 days. The reaction was centrifuged at 100,000 rpm, 1 hour, 4°C and then analysed on a Superdex 200 10/300GL column using 10 mM sodium phosphate pH 7.5 buffer with a flow rate of 0.5 mL/min. Proteins were detected at A280nm.