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. 2015 Feb 13;10(2):e0116497. doi: 10.1371/journal.pone.0116497

Fig 8. Differences between trypsin treatment of DA:α-syn 43–140 monomer and dimer.

Fig 8

A. Mass spectrum of the DA:α-syn 43–140 monomer after overnight digestion at 37°C by trypsin. A major mass at 1478.4 Da was observed corresponding to residues 81 to 95, while a mass at 1295.4 Da is consistent with a fragment between residues 46 to 58. B. Trypsin cleaves the DA:α-syn 43–140 dimer into a single detectable fragment. Mass spectrum of the trypsin digest of the DA:α-syn 43–140 dimer reveals a single peak of mass 1478.4 Da, and the absence (broken line box) of the 1295.4 Da fragment formed by the DA:α-syn 43–140 monomer digest. This suggests this fragment is potentially modified upon interaction with DA.