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. 2015 Feb 13;10(2):e0116497. doi: 10.1371/journal.pone.0116497

Fig 10. Truncation mutants of α-syn form oligomers after treatment with DA.

Fig 10

Truncated α-syn and mutants were treated with DA and the soluble fraction subjected to SDS-PAGE and visualised by silver staining A. Tricine SDS-PAGE. Lane 1: α-syn 43–140, Lane 2: α-syn 43–140 + DA, Lane 3: α-syn 43–60, Lane 4: α-syn 43–60 + DA. B. Lane 1: α-syn 43–140, Lane 2: α-syn 43–140 + DA (1:4), Lane 3: α-syn 43–140 + DA (1:10), Lane 4: α-syn 1–60, Lane 5: α-syn 1–60 + DA (1:4), Lane 6: α-syn 1–60 + DA (1:10). C. SEC of the α-syn 43–140 and α-syn 1–60 DA induced oligomers. 200 μM α-syn was incubated with 2 mM DA for 7 days. The reaction was centrifuged at 100,000 rpm, 1 hour, 4°C and then analysed on a Superdex 200 10/300GL column using 10 mM sodium phosphate pH 7.5 buffer with a flow rate of 0.5 mL/min. Proteins were detected at A280nm. The solid line represents α-syn 43–140 and broken line α-syn 1–60.