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. 2015 Feb 13;10(2):e0118295. doi: 10.1371/journal.pone.0118295

Fig 2. Repression of the vieSAB operon by H-NS and quorum sensing.

Fig 2

A. β-galactosidase activity produced by wild type (WT) and mutant strains containing an El Tor biotype vieSAB ET -lacZ promoter fusion. El Tor biotype strain C7258ΔlacZ and classical biotype strain O395ΔlacZ and their isogenic Δhns mutants (AJB80 and AJB608, respectively) were grown to stationary phase in LB medium. B. β-galactosidase activity produced by wild type (WT) and mutant strains containing a classical biotype vieSAB CL -lacZ promoter fusion. Strains C7258ΔlacZ and O395ΔlacZ and their isogenic Δhns mutants were grown to stationary phase in LB medium. C. Activity of the vieSAB ET -lacZ promoter fusion in wild type (C7258ΔlacZ), Δhns (AJB80), ΔhapR (AJB51ΔlacZ) and ΔhnsΔhapR (AJB700) mutants grown to stationary phase in LB medium. β-galactosidase activity (Miller units) was measured as an indicator of promoter activity. Each value represents the mean of six independent experiments. Error bars denote the standard deviation (STDEV).