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. 2015 Feb 13;10(2):e0118295. doi: 10.1371/journal.pone.0118295

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© 2015 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 7 — DIG-labeled DNA fragments containing the tcpA, vieSAB, tnaA, rtxBDE, rtxCA, hlyA, rpoE-P1, rpoE-P2 and VC1922 promoters were incubated with 0 (lane1), 10 (lane 2), 20 (lane 3) and 25 (lane 4 in tnaA panel) ng of H-NS protein. The spans of the promoter fragments used relative to the start codon were as follows: tcpA, -306 to-83; vieSAB, -331 to +21; tnaA, -480 to +70; rtxBDE, -188 to +39; rtxCA, -223 to +24; hlyA, -291 to +23; rpoE P1, -414 to-230; rpoE P2, -180 to-12; VC1922, -113 to +43. Lanes 4 in all panels except tnaA, and lane 5 in the tnaA panel contained a 100-fold excess of unlabeled competitor promoter DNA. The tcpA and VC1922 promoters were used as positive and negative controls, respectively.