Fig 9. Effect of HlyU on H-NS occupancy at the rtxCA, rtxBDE and hlyA promoters.

V. cholerae strain C7258HNS-FLAG containing pBAD-HlyU-His was grown to OD₆₀₀ 0.5 and the cultures divided in halves. One half was used as a control and the other half treated with 0.02% L-arabinose to induce HlyU expression. Cultures were incubated as described in materials and methods and the amount of promoter DNA IP with H-NS was quantitated by qPCR. Positive control, tcpA; negative controls, VC1922. Each value represents the mean of three experiments. Error bars denote the STDEV.