|
Strains
|
|
|
| C7258 |
Wild type O1; El Tor biotype |
Peru, 1991 |
| C7258ΔlacZ
|
C7258 lacZ deletion mutant |
[27] |
| AJB51ΔlacZ
|
C7258ΔlacZ ΔhapR
|
[27] |
| O395ΔlacZ
|
Classical biotype O395 lacZ deletion mutant |
[35] |
| AJB608 |
O395ΔlacZ Δhns::km |
This study |
| AJB80 |
C7258 ΔlacZ Δhns::km |
[27] |
| AJB700 |
C7258 ΔlacZ ΔhapR Δhns::km |
This study |
| C7258HNS-FLAG |
C7258 hns::hns-FLAG |
[25] |
| AJB606 |
C7258 ΔlacZΔvieA
|
This study |
| AJB607 |
C7258 ΔlacZ Δhns::km ΔvieA
|
This study |
| AJB600 |
C7258 ΔlacZ ΔvieSAB
|
This study |
| AJB601 |
C7258 ΔlacZ Δhns::km ΔvieSAB
|
This study |
| AJB602 |
C7258 ΔlacZ ΔhlyU::cm |
This study |
| AJB603 |
C7258 ΔlacZ Δhns::km ΔhlyU::cm |
This study |
| WL7258ΔlacZ |
C7258 ΔlacZ Δcrp
|
[27] |
| AJB604 |
C7258 ΔlacZ Δcrp Δhns::Km |
This study |
|
Plasmids
|
| pCR2.1 |
Bacterial expression vector for TA cloning (AmpR) |
Invitrogen |
| pBAD33 |
pACYC184 ori araC paraBAD, CmR
|
[45] |
| pBAD-HlyU-HIS |
pBAD33 containing the hlyU ORF 3’ expressed from the arabinose promoter |
This study |
| pCAT |
Chloramphenicol acetyl transferase (cat) gene in pUC219 |
B. Kan |
| pUC-VieA-UP |
0.56 kb XbaI-SalI DNA fragment 5’ of vieA in pUC19 |
This study |
| pUC-VieA-DN |
0.57 kb SalI-SphI DNA fragment 3’ of vieA in pUC19 |
This study |
| pUCΔVieA |
XbaI-SalI and SalI-SphI fragments flanking vieA in pUC19 |
|
| pCVDΔVieA |
XbaI-SphI fragment harboring vieA deletion in pCVD442 |
|
| pUC-VieSAB-UP |
0.52 kb XbaI-SalI DNA fragment 5’ of vieSAB in pUC19 |
This study |
| pUC-VieSAB-DN |
0.47 kb SalI-SphI DNA fragment 3’ of vieSAB in pUC19 |
This study |
| pUCΔVieSAB |
XbaI-SalI and SalI-SphI fragments flanking vieSAB in pUC19 |
This study |
| pCVDΔVieSAB |
XbaI-SphI fragment harboring vieSAB deletion in pCVD442 |
This study |
| pUC-HlyU-UP |
0.52 kb SacI-BamHI DNA fragment 5’ of hlyU in pUC19 |
This study |
| pUC-HlyU-DN |
0.48 kb BamHI-SphI DNA fragment 3’ of hlyU in pUC19 |
This study |
| pUCΔHlyU |
SacI-BamHI and BamHI-SphI fragments flanking hlyU in pUC19 |
This study |
| pUCΔHlyU- Cm |
0.8 kb BamHI fragment encoding cat gene in pUCΔHlyU |
This study |
| pCVDΔHlyU- Cm |
SacI-SphI fragment harboring vieSAB deletion and cat insertion in pCVD442 |
This study |
| pCVDΔHNSK |
DNA fragment carrying hns deletion and km insertion in pCVD442 |
[27] |
| pTT3 |
XbaI-PstI fragment encoding rrnBT
1
T
2 transcription terminator in pUC19 |
[38] |
| pTT3VieSABET
|
633 bp SphI-HindIII El Tot biotype vieSAB promoter fragment in pTT3 |
This study |
| pTT3VieSABCL
|
633 bp SphI-HindIII classical biotype vieSAB promoter fragment in pTT3 |
This study |
| pTT3HlyA |
540 bp SphI-HindIII hlyA promoter fragment in pTT3 |
This study |
| pTT3RtxCA |
250 bp SphI-HindIII rtxCA promoter fragment in pTT3 |
This study |
| pTT3RtxBDE |
230 bp SphI-HindIII rtxBDE promoter fragment in pTT3 |
This study |
| pTT3RpoEP1
|
190 bp SphI-HindIII rpoE P1 promoter fragment in pTT3 |
This study |
| pTT3RpoEP2
|
180bp SphI-HindIII rpoE P2 promoter fragment in pTT3 |
This study |
| pKRZ1 |
Plasmid source for promoterless lacZ gene |
[39] |
| pVieSABET-LacZ |
1040 bp KpnI-HindIII rrnBT
1
T
2-vieSAB
ET cassette ligated to promoterless lacZ gene in pKRZ1 |
This study |
| pVieSABCL-LacZ |
1040 bp KpnI-HindIII rrnBT
1
T
2-vieSAB
CL cassette ligated to promoterless lacZ gene in pKRZ1 |
This study |
| pHlyA-LacZ |
1001 bp KpnI-HindIII rrnBT
1
T
2-hlyA cassette fused to lacZ gene |
This study |
| pRtxCA-LacZ |
704 bp KpnI-HindIII fragment rrnBT
1
T
2-rtxCA cassette fused to lacZ gene |
This study |
| pRtxBDE-LacZ |
684 bp KpnI-HindIII rrnBT
1
T
2-rtxBDE cassette fused to lacZ gene |
This study |
| pRpoEP1-LacZ |
641 bp KpnI-HindIII rrnBT
1
T
2-rpoE
P1 cassette ligated lacZ gene |
This study |
| pRpoEP2-LacZ |
625 bp KpnI-HindIII rrnBT
1
T
2-rpoE
P2 cassette ligated to lacZ gene |
This study |
| pTT5 |
KpnI-BamHI rrnBT
1
T
2 transcription terminator fragment in pUC19 |
This study |
| pTT5TnaA |
1140 bp BamHI-XbaI tnaA promoter fragment in pTT5 |
This study |
| pTnaA-LacZ |
1570 bp KpnI-XbaI rrnBT
1
T
2-tnaA cassette ligated to lacZ gene |
This study |
| pCTX-LacZ |
rrnBT
1
T
2-ctxA promoter fragment ligated to lacZ gene |
[35] |
| pRpoE-LacZ |
rrnBT
1
T
2 and rpoE P1P2 promoter in tandem fused to lacZ gene |
[35] |
