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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Neuropharmacology. 2014 Dec 5;91:157–168. doi: 10.1016/j.neuropharm.2014.11.027

Figure 3.

Figure 3

An interaction involving the C loop β2 is needed for potentiation. Current responses evoked by ACh alone (squares; solid curves), ACh + 10 µM Mor (circles; dashed curves) and ACh + 10 µM Oxa (triangles; dotted curves) for three mutant receptor subtypes are plotted as a function of agonist concentration. A. Activation profiles for the double-cysteine receptor α3Y168Cβ2D190C are shown with black symbols and black fitted curves. Curves shown in red depict the best-fit parameters of the Hill equation for wild type α3β2 (symbols omitted; data from 32). B. Activation profiles for the single mutant α3Y168Cβ2 and α3β2D190C receptors are shown in black and red, respectively; the ACh concentrations for α3Y168Cβ2 are slightly offset for clarity. Symbols represent means (± SEM). Parameters for the Hill equation fits and replicates (n) are given in Table 1.