Table 2.

Composition of the 96-well additive screen

Well	Additive	Well	Additive
A1	water	E1	5 mM EDTA
A2	water	E2	100 mM sodium fluoride
A3	water	E3	100 mM potassium fluoride
A4	water	E4	100 mM lithium chloride
A5	100 mM Urea	E5	100 mM potassium chloride
A6	250 mM Urea	E6	100 mM ammonium chloride
A7	500 mM Urea	E7	100 mM sodium iodide
A8	1 M Urea	E8	100 mM potassium iodide
A9	25 mM Guanidine HCl	E9	100 mM sodium bromide
A10	50 mM Guanidine HCl	E10	10 mM magnesium chloride
A11	100 mM Guanidine HCl	E11	10 mM calcium chloride
A12	250 mM Guanidine HCl	E12	5 mM manganese chloride
B1	500 mM Guanidine HCl	F1	5 mM nickel chloride
B2	1% (v/v) DMSO	F2	5 mM iron (III) chloride
B3	2% (v/v) DMSO	F3	5 mM zinc chloride
B4	2.5% (v/v) Glycerol	F4	5 mM cobalt chloride
B5	5% (v/v) Glycerol	F5	100 mM sodium formate
B6	10% (v/v) Glycerol	F6	100 mM sodium acetate
B7	15% (v/v) Glycerol	F7	100 mM sodium malonate
B8	20% (v/v) Glycerol	F8	100 mM sodium nitrate
B9	2.5% (v/v) D-Glucose	F9	100 mM sodium thiocyanate
B10	5% (v/v) D-Glucose	F10	100 mM sodium sulfate
B11	2.5% (v/v) Sucrose	F11	100 mM ammonium sulfate
B12	5% (v/v) Sucrose	F12	100 mM ammonium chloride
C1	2.5% (v/v) PEG400	G1	2 mM AMP + 5 mM MgCl2
C2	5% (v/v) PEG400	G2	2 mM ADP + 5 mM MgCl2
C3	2.5% (w/v) PEG1000	G3	2 mM ATP + 5 mM MgCl2
C4	5% (w/v) PEG1000	G4	2 mM AMPPNP + 5 mM MgCl2
C5	2.5% (w/v) PEG4000	G5	2 mM cAMP + 5 mM MgCl2
C6	5% (w/v) PEG4000	G6	2 mM GDP + 5 mM MgCl2
C7	2.5% (v/v) Ethylene Glycol	G7	2 mM GTP + 5 mM MgCl2
C8	5% (v/v) Ethylene Glycol	G8	2 mM cGMP + 5 mM MgCl2
C9	1 mM Octyl Glucoside	G9	2 mM NAD + 5 mM MgCl2
C10	2 mM CHAPS	G10	2 mM NADH + 5 mM MgCl2
C11	10 mM L-Proline	G11	10 mM Betaine
C12	50 mM L-Glycine	G12	1 mM Spermine
D1	25 mM L-Histidine	H1	1 mM Spermidine (add fresh each time)
D2	50 mM L-Arginine	H2	10 mM β-mercaptoethanol (add fresh each time)
D3	50 mM L-Glutamate	H3	5 mM DTT (add fresh each time)
D4	50 mM L-Arg/50 mM L-Glu	H4	2 mM TCEP (add fresh each time)
D5	25 mM L-Glutamine	H5	open for user-determined additives
D6	50 mM L-Lysine	H6	open for user-determined additives
D7	50 mM L-Cysteine	H7	open for user-determined additives
D8	50 mM Taurine	H8	open for user-determined additives
D9	50 mM Imidazole pH 7.6	H9	open for user-determined additives
D10	100 mM Imidazole pH 7.6	H10	open for user-determined additives
D11	250 mM Imidazole pH 7.6	H11	open for user-determined additives
D12	500 mM Imidazole pH 7.6	H12	open for user-determined additives

Concentrations listed represent the final concentration in the thermal shift assay.

Stocks should be made at 5× concentration.