Fig 2.

Neurite analysis after exposure to conditioned BMDM media. (a–c) Representative images of primary DRG cultures immunolabelled for βIII-tubulin (green) and nuclei counterstained with Hoechst 32258 (blue). One-day-old DRG cultures were treated for 24 h with DMEM/F-12 supplemented 1:1 with culture media from BMDMs stimulated for 4 h prior with (a) vehicle, (b) 2 nM denatured-gp120_Bal, or (c) 2 nM gp120_Bal. Scale bars=50 µm. DRG cultures treated with culture media from gp120_Bal-treated BMDMs showed a significant reduction in the mean NOPN, as a percentage of vehicle-treated cultures (d). Conditioned media from denatured-gp120_Bal-treated BMDMs induced no change in neurite outgrowth relative to vehicle control. Data presented as mean (sd). *P<0.05 vs vehicle-treated cultures, using one-way anova and Tukey's post hoc analysis (n=5–6; five to six technical replicates across two biological studies).