Fig 4.

Effect of TNF-α on neurite outgrowth in primary adult DRG cultures. Representative images of adult primary DRG cultures treated for 24 h with vehicle (a) or 0.25 nM (b), 0.5 nM (c), 1 nM (d), and 2 nM (e) TNF-α in C-DMEM. Addition of TNF-α induced reduced neurite outgrowth that was significant at 2 nM (f). Data presented as mean neurite outgrowth/neurone (sd), after 24 h treatment, normalized to (percentage of) the mean neurite outgrowth/neurone of vehicle-treated cultures (n=3–9). *P<0.05 using one-way anova and Tukey's post hoc analysis. Scale bars=50 µm.