Table 2.

PCR conditions and primer sequences for the three markers sequenced in this study.

Marker	Primer sequences and references	PCR recipe (µL); 25-µL aliquots	Cycler programme
ETS	ETS Salicornia-F 5′-GTCCCTATTGTGTAGATTTCAT-3′; 18S-II R 5′-CTCTAACTGATTTAATGAGCCATTCGCA-3′ (Kadereit et al., 2007)	ddH2O 16·3, MgCl2 (50 mg mL−1) 2·25, buffer 2·5, dNTPs (10 µm) 0·25, Taq polymerase 0·2, F primer (10 µm) 1, R primer (10 µm) 1, DNA template 1·5	95 °C for 4 min, 30 cycles of [95 °C for 30 s, 50·5 °C for 45 s, 72 °C for 4 min], 50·5 °C for 72 s, 72 °C for 8 min
			or 97 °C for 90 s, 35 cycles of [97 °C for 20 s, 69 °C for 90 s, 72 °C for 90 s], 72 °C for 7 min
rpL32–trnL spacer	trnL (UAG): 5′-CTGCTTCCTAAGAGCAGCGT-3′; rpl 32-F: 5′-CAGTTCCAAAAAAACGTACTTC-3′ (Shaw et al., 2007)	ddH2O 19·45, MgCl2 (50 mg mL−1) 0·6, buffer 2·5, dNTPs (10 µm) 0·25, Taq polymerase 0·2, F primer (10 µm) 0·5, R primer (10 µm) 0·5, DNA template 1·0	94 °C for 60 s, 35 cycles of [94 °C for 30 s, 52 °C for 50 s, 72 °C for 60 s], 94 °C for 30 s, 52 °C for 72 s, 72 °C for 8 min
atpB–rbcL spacer	atpB–rbcL-spacer F 5′-GAAGTAGTAGGATTGATTCTC-3′; atpB–rbcL-spacer R 5′-CAACACTTGCTTTAGTCTCTG-3′ (Kadereit et al., 2006)	ddH2O 19·25, MgCl2 (50 mg mL−1) 1, buffer 2·5, BSA 0·25, dNTPs (10 µm) 0·25, Taq polymerase 0·25, F primer (10 µm) 0·5, R primer (10 µm) 0·5, DNA template 0·5	94 °C for 1 min, 35 cycles of [94 °C for 20 s, 56 °C for 30 s, 72 °C for 60 s], 94 °C for 20 s, 56 °C for 80 s, 72 °C for 8 min