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| Pedigree | Primer Name | Primer Sequence(5′-3′) | Size(bp) | Annealing T(°C) |
|---|---|---|---|---|
| X | XF | ATGTGACAGCCGGTGATGAG | 102 | 55 |
| XR | TGGGAGAAGAAGCCAACAGC | |||
| H | HF | AAAGGATAATTTGGAAGGTTTCTG | 421 | 55 |
| HR | TGTGACTTAAATGTTTACTCTCTCTCC |
RFLP, restriction fragment length polymorphism
