Table 5. Predicted effects due to the mutations in MYBPC1 using SIFT and PolyPhen-2.

Variants	Domain	SIFT	PolyPhen-2	Memo
p.P319L	C2	Tolerated(0.29)	Possibly damaging(0.593)	reported in the present study
p.E359K	C2	Tolerated(0.11)	Probably damaging(0.999)	reported in the present study
p.W236R	M motif	Tolerated(0.43)	Probably damaging(0.999)	[12]
p.Y856H	C8	Tolerated(0.31)	Probably damaging(0.997)	[12]

SIFT: scores % less than 0.05 indicate substitutions are predicted as intolerant.

PolyPhen-2: scores are evaluated as 0.000 (most probably benign) to 0.999 (most probably damaging).