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. 2015 Feb 13;9(2):e0003476. doi: 10.1371/journal.pntd.0003476

Fig 5. Subtelomeric deletion of chromosome 31 and LgAQP1 expression in antimony resistant L. guyanensis.

Fig 5

(A) Zoomed representation of raw read depth for one of the subtelomeric region of chromosome 31. The inset scheme indicates the gene positions on the chromosome. Black, LgM4147 WT; Blue, LgSbIII650.1; Red, LgSbIII650.2; Green, LgSbIII650.3; and Yellow, LgSbIII650.4. (B) Southern blot hybridization validating the subtelomeric deletions of chromosome 31. PstI-digested genomic DNAs were hybridized with probes derived from genes located within (LbrM.31.0010—LbrM.31.0070) or outside (LbrM.31.0100) the deleted region. GAPDH was used as a qualitative DNA loading control for one of the blots and should not be used for determining changes in gene copy numbers. Lanes: 1, LgM4147 WT; 2, LgSbIII650.1; 3, LgSbIII650.2, 4, LgSbIII650.3; 5, LgSbIII650.4. (C) Relative AQP1 mRNA levels in LgSbIII650.1, LgSbIII650.2, LgSbIII650.3 and LgSbIII650.4 and their revertants compared to WT. Revertants were cultured for at least 26 passages in the absence of SbIII. The SbR/WT expression ratios were normalized according to GAPDH (LbrM.30.2950) levels. Values are the mean of at least three independent experiments each performed with three biological replicates.