Table 1. Half-maximal effective concentrations (EC50) of SbIII and correspondent resistance indexes (RI) for L. guyanensis.
| Strain | SbIII EC50 (μM) ± SEM | RI |
|---|---|---|
| L guyanensis M4147 WT | 53.72 ± 0.12 | |
| LgSbIII650.1 | >1000 | >18.6 |
| LgSbIII650.2 | >1000 | >18.6 |
| LgSbIII650.3 | >1000 | >18.6 |
| LgSbIII650.4 | >1000 | >18.6 |
| LgSbIII650.1 rev | >1000 | >18.6 |
| LgSbIII650.2 rev | >1000 | >18.6 |
| LgSbIII650.3 rev | >1000 | >18.6 |
| LgSbIII650.4 rev | >1000 | >18.6 |
| LgSbIII80.1/2013 | 318.4 ± 2.95 | 6 |
| LgSbIII80.2/2013 | 221.8 ± 2.25 | 4.12 |
| LgSbIII160.1/2013 | 450.5 ± 2.87 | 8.4 |
| LgSbIII160.2/2013 | >1000 | >18.6 |
| LgSbIII240.1/2013 | 853.4 ± 3.4 | 15.8 |
| LgSbIII240.2/2013 | 966.3 ± 4.6 | 18 |
| LgSbIII325.1/2013 | >1000 | >18.6 |
| LgSbIII325.2/2013 | >1000 | >18.6 |
| LgSbIII650.1/2013 | >1000 | >18.6 |
| LgSbIII650.2/2013 | >1000 | >18.6 |
| LgSbIII650.2 + pSP72αZEOαLgAQP1WTGFP | 29.09 ± 0.35 | 0.54 |
| LgSbIII650.2 + pSP72αZEOαLgAQP1G133DGFP | >1000 | >18.6 |
| LgSbIII650.2 + pSP72αZEOα | >1000 | >18.6 |
| LgSbIII650.2 + pSP72αZEOαLgAQP1WT | 31.4 ± 0.57 | 0.6 |
| LgWT + pSP72αZEOαLgAQP1WT | 12.93 a ± 0.32 | 0.24 |
| LgWT + pSP72αZEOαLgAQP1G133D | 51.98 ± 0.33 | 0.96 |
| LgSbIII650.4 + pSP72αZEOαLgAQP1WT | 28.63 b ± 0.38 | 0.53 |
SEM (standard error of the mean); RI (resistance index)
a 76% increased SbIII sensitivity compared to Lg WT
b At least 97% re-sensitization when compared to LgSbIII650.4
EC50 values are the average of at least three independent experiments.