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Cross-peak clusters are formed for many methyl groups upon PIDDosome formation. Selected regions of the 13C-methyl-TROSY NMR spectra of ILV-13CH3-labeled RAIDD-DD (a and c) and PIDD-DD (b) in the presence (red) and absence (black) of excess unlabeled PIDD-DD and RAIDD-DD, respectively, plotted at low contour threshold to reveal the clusters of low-intensity cross-peaks associated with PIDDosome formation. Methyl-group assignments of the cross-peaks in the unbound state are indicated.
