Fig. 9.

Comparison of the ¹³C-methyl-TROSY NMR spectra of wild type (a and c) and RAIDD-DD (E188K) (b and d) in the absence and presence of excess PIDD-DD. The positions of the cross-peaks of unbound RAIDD-DD (E188K) are essentially identical with those of the wild type. However, notable differences in the cross-peak clusters corresponding to the well-resolved RAIDD-DD Val189-C^γ2H₃ cross-peak (highlighted by red rectangles) are detected, presumably reflecting both the proximity to the mutation site at residue 188 and the different overall stoichiometry of the complex formed.