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Non-equivalence of methyl-group side chains (gray) among the pseudoequivalent interdomain contacts in the PIDDosome core crystal structure (PDB code 2OF5) is reflected in the distribution of the transverse relaxation optimized spectroscopy nuclear magnetic resonance intensity observed as a single feature for the isolated PIDD death domain (DD) (black contours) into multiple features in the spectrum of the complex (red contours).
