Fig. 4.

DIC or OGC do not transport GSH (A) [³⁵S]-GSH uptake by fused membranes of the control (white bars), OGC-expressing (grey bars) and DIC-expressing (black bars) strains. The fused membranes were internally loaded with 5 mM of the indicated substrates (in) and incubated with 1.5 μM of the indicated external radio-labelled substrate (out) for 15 min. The initial exchange rates were determined over the first 60 s. (B) [³⁵S]-GSH uptake at different pH values. Fused membranes of the control (white bar), OGC-expressing (grey bars) and DIC-expressing (black bars) strains were preloaded with 5 mM GSH and incubated with 1.5 μM [³⁵S]-GSH to initiate exchange. The initial exchange rates were determined in the first 60 s time interval. Data are represented by the mean ± S.D. (n = 4). ^∗∗∗P < 0.001 for comparison of transport of radiolabelled substrate by control vesicles against OGC/DIC vesicles by ANOVA.