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. 2015 Feb 11;8:40. doi: 10.1186/s13104-015-0997-z

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© Ghosh et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Detection of elongation factor 1 alpha gene of Fusarium oxysporum f. sp. ciceris by LAMP. (A) LAMP detection using HNB as a visual indicator. The reaction becomes sky blue if the target gene is present (Tube 2) but remains violet if the gene is absent (Tube 1). (B) Agarose gel electrophoresis of LAMP products. The positive reaction is manifested as a ladder-like pattern on the 1.5% agarose gel. M = 100 bp DNA marker. Lane1: Positive reaction (with target DNA). Lane B: Control (without template DNA).