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. 2015 Feb 11;8:40. doi: 10.1186/s13104-015-0997-z

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© Ghosh et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Optimization of LAMP reaction time and reaction temperature. (A,B) Optimization of time, (A) Assessment was based on 1.5% agarose gel electrophoresis and (B) HNB visualization of colour change. Lane 1: 15 min; lane 2: 30 min; lane 3: 45 min; lane 4: 60 min; and lane 5: 90 min. (C,D) Optimization of temperature, (C) Assessment was based on 1.5% agarose gel electrophoresis and (D) HNB visualization of colour change. Lane 1: 63°C; lane 2: 61°C; lane 3: 59°C; and lane 4: 57°C. M indicates a 100 bp DNA marker.