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. 2015 Feb 11;8:40. doi: 10.1186/s13104-015-0997-z

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© Ghosh et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparative sensitivity of LAMP and conventional PCR analysis for detection of Fusarium oxysporum f. sp. ciceris using serial dilutions of DNA as template. A) Electrophoresis analysis of LAMP. Lane 1: 10 μg; lane 2: 1 μg; lane 3: 100 ng; lane 4: 10 ng; lane 5: 1 ng; lane 6: 100 pg; lane 7: 10 pg; lane 8: 1 pg; lane 9: 100 fg; lane 10: 10 fg. Lane M, 100 bp DNA Marker. B) Electrophoresis analysis of conventional PCR with ITS-1 and ITS-4 primers. Lane M, DNA marker (1 Kb). C) Visual detection of LAMP assay by addition of HNB.