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. Author manuscript; available in PMC: 2015 Feb 19.
Published in final edited form as: Am J Clin Pathol. 2014 Jun;141(6):856–866. doi: 10.1309/AJCPMWGWGO34EGOD

Figure 2.

Figure 2

Background noise. For each of the common mutations within KRAS (codons 12 and 13), BRAF (V600E), and EGFR (T790M and L858R) genes among the 16 formalin-fixed, paraffin-embedded (FFPE) lymph node control first library (A), a second FFPE library (B), and peripheral blood (C) specimens, the mean plus 3 SD is plotted. Note that the patterns of quiet bases and noisy bases in A and B are qualitatively similar and that background signal in FFPE is substantially higher than in the peripheral blood. Down arrows designate consistently quiet mutations. Asterisks indicate particularly noisy mutations that have arisen due to a C:G→T:A mutation (resulting in either a C→T or a G→A on the sense strand).