Fig. 5.

Evaluation of the internalization of two-component delivery system driven by in situ click reactions with confocal fluorescent microscopy. (A) Albumin-based carrier without paclitaxel (Alb-1). Treated: BT-474 cells were treated with Tz20 (trastuzumab functionalized with azide), followed by Alb-1 at 37 °C Control: BT-474 cells were treated with Tz-1 (trastuzumab without azide), followed by Alb-1 at 37 °C. (B) Albumin-based carrier conjugated with paclitaxel (Alb2.2). Treated: BT-474 cells treated with Tz20, followed by Alb2.2 at 37 °C Control: BT-474 cells treated with Tz-1, followed by Alb2.2 at 37 °C