Fig. 3. MJ-66 has no effect on non-proliferating cell death.
A. Concentration-dependent effects of MJ-66 on human normal glia cell line and rat primary glia cells. Cells were treated with MJ-66 at various times as indicated. B. C6 glioma cells were treated with Ara-C (41.6 nM) for the indicated times and cell cycle distributions were monitored by flow cytometry with propidium iodide staining. C. Upper panel: C6 glioma cells were treated with MJ-66 (60 nM) for the indicated times and cell cycle distributions were monitored by flow cytometry with propidium iodide staining. Middle and Lower panels: C6 glioma cells were pre-treated with Ara-C (41.6 nM) for 12 h and were treated with MJ-66 (60 nM) for the indicated times. Cell cycle distributions were monitored by flow cytometry with propidium iodide staining.