Skip to main content
. Author manuscript; available in PMC: 2015 Feb 19.
Published in final edited form as: Mol Imaging. 2012 Nov-Dec;11(6):499–506.

Figure 1.

Figure 1

Time course of BLI signal in 22Rv1 cells treated with HhAntag-691 (A) or fumitremorgan C (FTC) (B). Cells were transiently transfected with a cytomegalovirus promoter–controlled trifusion reporter gene that produces fLuc, red fluorescent protein (RFP), and a mutated form of herpes simplex virus 1 thymidine kinase (sr39ttk), as previously described.4 HhAntag-691 or FTC was added at the indicated concentration, followed by D-luciferin, which was at a final concentration of 150 mg/mL. Imaging commenced immediately after adding D-luciferin. HhAntag-691 caused a transient, dose-dependent signal decrease before a dose-dependent signal increase became evident, whereas FTC caused enhanced BLI signal from the outset.